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. Author manuscript; available in PMC: 2013 Sep 25.
Published in final edited form as: Mol Cell Endocrinol. 2012 Mar 28;361(1-2):31–39. doi: 10.1016/j.mce.2012.03.008

Figure 1. Effect of PMA on 1,25(OH)2D3-regulated expression of CYP24A1 and TRPV6 mRNA in differentiated Caco-2 cells.

Figure 1

Differentiated Caco-2 cells were pretreated with vehicle (DMSO) or 100 nM PMA for 5 min followed by treatment with vehicle (EtOH) or 10 nM 1,25(OH)2D3 for an additional 2 h in the absence of PMA. Total RNA was collected and analyzed by real time RT-PCR for (A) CYP24A1 mRNA and (B) TRPV6 mRNA. Data are expressed as the mean±sem (n=4). Bars with different letter superscripts are significantly different from one another (p < 0.05, Fisher’s protected LSD). Data in each panel are representative of three independent experiments.