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. Author manuscript; available in PMC: 2013 Sep 25.
Published in final edited form as: Mol Cell Endocrinol. 2012 Mar 28;361(1-2):31–39. doi: 10.1016/j.mce.2012.03.008

Figure 6. Effect of PMA on Sp3 serine phosphorylation in proliferating Caco-2 cells.

Figure 6

Differentiated Caco-2 cell were treated with vehicle (DMSO) or PMA (5 min, 100 nM) and then harvested. An additional group of cells were pretreated with U0126 (10 µM, 30 min) prior to PMA treatment. Sp3 was immunoprecipitated from the cell extracts and examined by Western blot analysis for total Sp3 level (L1, L2, M1 and M2 are Sp3 isoforms) and for phospho-serine. (A) Representative Western blot. The phospho-serine band corresponds to the L1 Sp3 isoform, (B) Quantitative data from three repeat experiments. Bands were quantified by densitometry and phospho-serine labeled Sp3 (L1) was normalized to the total amount of Sp3 L1 isoform present. Data are expressed as mean±sem (n=3) of the fold change above non-treated levels. * Significantly different from the other two groups (p < 0.05, Fisher’s protected LSD).