Figure 6.

A lipid droplet is more likely to continue moving in its original direction after the trap detaches the motors from the microtubule. (A) A lipid droplet trapped at multiple positions along its trajectory shows successive excursions and detachments in the plus-end direction. (B) An enlarged section of the trajectory in A. (C) Excursions in the optical trap after a stall are counted and classified as either plus-end, minus-end, or no motion. Plus stalls are more likely to be followed by plus-end-directed motion and minus stalls are more likely to be followed by minus-end motion. (The total number of plus transitions is 358 and minus transitions is 186, the maximum waiting time after a stall was 0.5 s for an excursion to be counted).