Figure 2.

Thermal transition curves of the WT RMCK, C101 and C113 by the mean residue molar ellipticity at 222 nm (A) and I₃₂₀/I₃₆₅ of the Trp fluorescence spectra (B). The transition curves were fitted by Eq. 2, and the thermodynamic parameters are listed in Table S2. For the transition curve of WT RMCK from CD spectroscopy, both two-state and three-state models were applied for fitting. The three-state fitting was performed by regarding the transition as the sum of two sequential two-state processes because a stable intermediate could be observed.