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Mutational analysis of the role of Phe-250, Val-255, and Val-347 in RMCK thermal unfolding and aggregation. (A–C) Thermal aggregation of C113 (A), C101 (B), and RMCK (C) at 46°C. (D–F) Comparison of the kinetic parameters of thermal aggregation. (G) Thermal unfolding transition curves of the WT RMCK and the mutants. The raw data (symbols) were fitted by Eq. 2, and the fitted data are presented as solid lines.
