Figure 4.

Ionic strength effect on Bax channel dynamics is not mediated by tBid. Mitochondrial suspensions were incubated in 10 mM KCl buffer (solid line) or 90 mM KCl buffer (dashed line) with 34 nM Bax and varying concentrations of tBid (24, 60, and 120 nM) for 30 min at 30°C. Then, the real-time permeability was determined by measuring the cytochrome c oxidation rate. There was no significant difference in cytochrome c oxidation rates between any treatments at 90 mM KCl buffer. The results are mean ± SE of three experiments.