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Two-dimensional 15N-1H-HSQC spectra from CaM-MA. (a) 15N-MA only; (b) 15N-MA-CaM complex in 10 mM NaPO4 (pH 5.5), 5 mM CaCl2, 1 mM TCEP; (c and d) the 15N-MA-CaM complex after titration with either KCl (to 500 mM) or EGTA (10 mM), respectively, showing many peaks corresponding to folded MA have been restored; (e) 15N-MA in 6 M GuHCl, and (f) 15N-MA after removal of 6 M GuHCl.
