Table 3.
Ion localization inside the cytoplasmic domain: SPM4+ + ∼1 M KCl simulations
| Kir1.1/ROMK |
Kir2.1/IRK |
|||||
|---|---|---|---|---|---|---|
| SPM4+ | K+ | Cl− | SPM4+ | K+ | Cl− | |
| Central pore (R < 15 Å, −9 Å < Z < 15 Å) | 4.0 ± 0.0 | 5.9 ± 0.4 | 2.0 ± 0.6 | 3.7 ± 0.3 | 4.7 ± 1.4 | 1.4 ± 0.1 |
| PIP2 binding region (5 Å < R < 30 Å, 15 Å < Z < 30 Å) | 0.0 ± 0.0 | 11.5 ± 0.5 | 30.4 ± 0.8 | 0.0 ± 0.0 | 11.7 ± 0.2 | 26.9 ± 0.7 |
| Constriction region (R < 15 Å, 15 Å < Z < 25 Å) | 0.0 ± 0.0 | 1.5 ± 0.2 | 1.3 ± 0.1 | 0.0 ± 0.0 | 0.8 ± 0.1 | 1.0 ± 0.1 |
| Cytoplasmic entrance (R < 20 Å, −30 Å < Z < −10Å) | 0.0 ± 0.0 | 11.7 ± 1.1 | 9.0 ± 0.6 | 0.2 ± 0.2 | 23.0 ± 0.3 | 8.2 ± 0.4 |
| Bulk (R < 40 Å, 35 Å < Z < 40 Å) | 0.0 ± 0.0 | 16.3 ± 0.2 | 16.7 ± 0.3 | 0.0 ± 0.0 | 13.7 ± 1.8 | 13.8 ± 1.8 |
All numbers are represented as averages ± SD (n = 3).