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. 2012 Aug 8;103(3):532–540. doi: 10.1016/j.bpj.2012.06.034

Figure 2.

Figure 2

VSMCs were exposed to agLDL (50 μg/mL) for increasing periods of time (8, 16, 24, 48, and 96 h). Real-time PCR quantification of tropoelastin mRNA levels was performed. Data were processed with a specially designed software program based on Ct values, normalized to GAPDH mRNA, and expressed as the mean ± SE of three independent experiments performed in duplicate.