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AFM images of silk fibroin after different treatments: (a) fresh silk fibroin solution; (b) sample (a) incubated at 60°C for 8 hr; (c) sample (a) slowly concentrated at 60°C for 3 days. In order to avoid masking the original morphology by multilayers of silk fibroin, all samples were diluted to below 0.1 wt% before investigation.
