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. Author manuscript; available in PMC: 2012 Aug 9.
Published in final edited form as: Methods Enzymol. 2010;476:53–69. doi: 10.1016/S0076-6879(10)76004-3

Figure 4.3.

Figure 4.3

(A) Cooling of loaded straws in a Styrofoam box. The Styrofoam box must be covered with a matching lid (not shown) during the cooling period. (B) A metal cane with attached plastic goblets. After freezing, the straws are placed inside the bottom goblet, and then the top goblet is sliding down to completely enclose the straws, to prevent the straws from falling out. Holes are made on the closed end of the top goblet to let air out and LN2 into the goblet.