ApoE−/−/Gclm−/− Mice (A) Male mice at 20 weeks (apoE−/−/Gclm−/− n=5, apoE−/−/Gclm+/+ n=17), 30 weeks (apoE−/−/Gclm−/− n=8, apoE−/−/Gclm+/+ n=13), 40 weeks (apoE−/−/Gclm−/− n=7, apoE−/−/Gclm+/+ n=10) and 50 weeks (apoE−/−/Gclm−/− n=9, apoE−/−/Gclm+/+ n=4); (C) female mice at 20 weeks (apoE−/−/Gclm−/− n=5, apoE−/−/Gclm+/+ n=24), 30 weeks (apoE−/−/Gclm−/− n=8, apoE−/−/Gclm+/+ n=14), 40 weeks (apoE−/−/Gclm−/− n=6, apoE−/−/Gclm+/+ n=10) and 50 weeks (apoE−/−/Gclm−/− n=5, apoE−/−/Gclm+/+ n=20). (E) Aortic sinus of female mice at 40 weeks of age (apoE−/−/Gclm−/− n=5, apoE−/−/Gclm+/+ n= 5)
ApoE−/−/Gclc-Tg Mice. (B) Male mice at 20 weeks (apoE−/−/Gclc-Tg n=11, apoE−/−/Gclc-WT n=12), 30 weeks (apoE−/−/Gclc-Tg n=7, apoE−/−/Gclc-WT n=6), 40 weeks (apoE−/−/Gclc-Tg n=6, apoE−/−/Gclc-WT n=11) and 50 weeks (apoE−/−/Gclc-Tg n=7 apoE−/−/Gclc-WT n=13); (D) Female mice at 20 weeks (apoE−/−/Gclc-Tg n=14, apoE−/−/Gclc-WT n=15), 30 weeks (apoE−/−/Gclc-Tg n=15, apoE−/−/Gclc-WT n=12), 40 weeks (apoE−/−/Gclc-Tg n=7, apoE−/−/Gclc-WT n=7) and 50 weeks (apoE−/−/Gclc-Tg n=8, apoE−/−/Gclc-WT n=18); (F) Aortic sinus of male mice at 50 weeks of age (apoE−/−/Gclc-Tg n=5, apoE−/−/Gclc-WT n=5). Data are presented as means ± SE, *p<0.05 vs controls.