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. Author manuscript; available in PMC: 2012 Aug 9.
Published in final edited form as: J Immunol. 2012 Jun 1;189(1):269–278. doi: 10.4049/jimmunol.1200365

Figure 4.

Figure 4

When tested at 5 days p.i., RT-PCR confirmed that silencing AC7 vs scrambled control treatment significantly lowered AC7 mRNA levels (A) and silencing together with VIP treatment did not reverse the effect. When tested at 5 days p.i., silencing AC7 increased mRNA levels of TLR1 (B), while TRAF6 (C) and ST2 (D) were decreased. Silencing together with VIP injection did not change the effect of silencing for any of the above mentioned molecules. In contrast, Chuk (E), IRAK1 (F), TLR4 (G), TLR9 (H) and IRAK2 (I) mRNA levels were increased after silencing AC7 vs scrambled control treatment. Silencing together with VIP injection decreased mRNA expression of each of these molecules significantly. SIGIRR mRNA levels (J) were decreased after silencing AC7, but together with VIP treatment, mRNA levels increased over silencing alone. No significant changes in mRNA levels of TLR6 (K) or TLR8 (L) were seen with AC7 siRNA treatment, but together with VIP treatment, mRNA levels were reduced only for TLR6.