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. Author manuscript; available in PMC: 2012 Aug 9.
Published in final edited form as: Nat Methods. 2011 Jul 10;8(8):671–676. doi: 10.1038/nmeth.1648

Figure 5. High-throughput siRNA kinome screen to identify modifiers of engineering outcome.

Figure 5

(a) Scatter plot of gene targeting and mutNHEJ Z scores obtained from the siRNA screen. Library in gray, Control siRNAs in black. Control siRNA values are average of at least 3 independent transfections. (b) Gating analysis comparing TLR readout from control and DNA-PKcs siRNA treatment as a function of nuclease expression 72hrs after transduction of HEK293 TLRsce I-SceI-T2A-IFP LV. Inset histogram shows nuclease expression gates. Data are derived from 3 independent experiments performed in duplicate.