Figure 5.

The changes in VEGF mRNA levels induced by PKCβ activation depend on the cell type and the culture condition. A-B: Determination of the levels of VEGF mRNA with quantitative real time RT–PCR in the pericytes (PC; A) and endothelial cells (EC; B). The measures of the total VEGF mRNA were normalized in accordance with the corresponding levels of RPL10a mRNA. The values are expressed as mean±SEM. The comparisons were performed between control (CTR) and PMA-treated (PMA) cells in monoculture (_mono) and in coculture (_cocu). The experiments were performed on three distinct sets of cells. *p<0.01; **p<0.005, n=5.