Figure 5.

Immunohistochemical staining of the colon and distal ileum. Specimens of mouse colon and ileum were studied immunohistochemically for the presence of CXCR4 (integral membrane proteins). Sections labeled with goat antirabbit immunoglobulin G horseradish peroxides and developed with 3,3′-diaminobenzidine produced a brown color and were counterstained with hematoxylin (blue color). Immunohistochemical staining of the colon and distal ileum showed strong immunoreactivity in the colon and distal ileum in group A. Arrows show CXCR4 protein (magnification 40×) (A). Moderate immunoreactivity was detected in the colon and distal ileum in group D. Arrows show CXCR4 protein (magnification 40×) (B). Faint immunoreactivity was detected in the colon in group E (magnification 40×) (C). Faint to negative immunoreactivity was detected in the colon in group F (magnification 40×) (D).