Table 1.
Average and maximum charge states for crosslinked cytochrome c and ubiquitin as a function of m-NBA concentration in the electrospray solution.
| Number of Crosslinks |
Average (Maximum) Charge |
|||
|---|---|---|---|---|
| Cytochrome c | % m-NBA | |||
| BS3 | 0 | 0.5 | 1.0 | 1.5 |
|
|
||||
| 2 | 7.0 (8) | 8.5 (11) | 10.0 (12) | 10.3 (13) |
| 3 | 7.0 (8) | 8.4 (11) | 9.7 (12) | 10.0 (13) |
| 4 | 6.9 (8) | 8.2 (11) | 9.3 (12) | 9.7 (13) |
| 5 | 6.9 (8) | 8.0 (11) | 8.9 (12) | 9.2 (13) |
| 6 | 6.9 (7) | 7.8 (9) | 8.4 (9) | 8.9 (12) |
| 7 | 7.0 (7) | 7.6 (8) | 7.9 (8) | 8.2 (9) |
| DEB | ||||
| 2 | 7.0 (8) | 7.5 (9) | 9.9 (12) | 9.8 (12) |
| 3 | 6.9 (8) | 7.6 (9) | 9.9 (12) | 9.9 (12) |
| 4 | 6.9 (8) | 7.5 (9) | 9.7 (12) | 9.6 (12) |
| 5 | 6.9 (8) | 7.4 (9) | 9.1 (10) | 9.4 (11) |
|
Ubiquitin
BS3 |
||||
| 0 | 5.6 (6) | 8.4 (11) | 8.5 (11) | 8.8 (11) |
| 1 | 5.5 (6) | 7.8 (10) | 7.7 (10) | 7.9 (10) |
| 2 | 5.8 (6) | 6.4 (7) | 6.5 (7) | 6.6 (7) |