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. 2012 Aug 9;7(8):e42828. doi: 10.1371/journal.pone.0042828

Figure 2. Effects of a gamma-secretase inhibitor on apoptosis-related genes Bax and Bcl-2 in the dentate gyrus of adult ischemic rats in response to chronic mild stress.

Figure 2

Western blotting analysis with anti-Bax and anti-Bcl-2 was performed to quantify protein levels of Bax (A), Bcl-2 (B) and ratio of Bax to Bcl-2 (C) in response to chronic mild stress and the effects of gamma-secretase inhibitor DAPT on Day 28 after ischemia surgery. Real-time PCR was also performed to investigate gene expression of Bax (D), Bcl-2 (E) and ratio of Bax to Bcl-2 (F) in response to chronic mild stress and the effects of gamma-secretase inhibitor DAPT on Day 28 after ischemia surgery, number of each group = 6. Representative photographs from three to four independent experiments are shown. Statistically significant differences between CMS, MCAO and MCAO+CMS compared with CON are indicated (# P<0.05), MCAO+CMS compared with MCAO are indicated (▴ P<0.05), or MCAO+CMS+D compared with MCAO+CMS are indicated (★ P<0.05), as determined by Bonferroni correction. CON, Control; CMS, Chronic mild stress; MCAO, Middle cerebral artery occlusion; D, DAPT (N-[N-(3,5-difluorophenacetyl-L-alanyl)]-S-phenyl-glycine t-butyl ester).