Figure 2. Endonuclease target site and chromosomal distribution of recovered Alu inserts.

A. The rescued Alu insert target consensus site matches the known L1 endonuclease site. Sequence logos [83] representing genomic pre-integration site consensus sequences of the rescued Alu inserts driven by full-length L1, L1 ORF2 alone, and all Alu inserts combined are shown. Previously published data for de novo L1 inserts recovered from culture [9] is shown for comparison. The arrow indicates the endonuclease cleavage site. B. Chromosomal distribution of de novo Alu inserts. Histogram of the chromosomal distribution of recovered de novo Alu inserts is shown. Note that chromosome Y was not included as all data were generated using the female cell line HeLa. C. Ideogram shows chromosomal locations of Alu inserts driven by a full-length L1 (red pins) and by ORF2 alone (blue pins).