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. 2012 Aug 9;8(8):e1002871. doi: 10.1371/journal.ppat.1002871

Figure 1. Vector constructs used to establish stable transgenic lines of Strongyloides ratti incorporate elements of the piggyBac transposon system.

Figure 1

A) Donor vector pPV254 incorporating a fluorescent reporter gene in which expression of GFP is driven by the Ss-act-2 promoter and terminated by the Ss era-1 3′ UTR. The reporter transgene in pPV254 is flanked by the inverted terminal repeats (ITR) plus internal sequences common to piggyBac transposable elements. B) Donor vector pPV356, which is like pPV254 in all respects except that the coding sequence is flanked by the gypsy retroviral insulator sequences from Drosophila. C) Helper vector pPV402 in which expression of the piggyBac transposase gene is driven by Ss-rps-21 promoter and terminated by the Ss-era-1 3′ UTR. D) Plasmid pPV257 for in vitro transcription of mRNA encoding the piggyBac transposase under the T7 promoter. In lieu of helper vector pPV402, this mRNA was capped, tailed and co-injected with donor vector pPV356 in Experiment 2.