Table 2. Genomic integration sites for reporter transgenes and their flanking sequences in three stable transgenic lines of Strongyloides ratti.
Stable line | Donor vector | Insertion | Integration junction accession | Contig | Chr.a | Location | 5′ sequence junction | Target site | 3′ sequence junction |
PV2 | pPV356 | PV2-1 | JX070935 | 74278 | I | 172601 | ACTTTAACGTTGTCATGATTTTTTT | TTAA | ATTTCATTATACTATTTTAAAAGTA |
“ | pPV356 | PV2-2 | JX070931 | 74996 | I | 155901 | GGTGGTGGAGATAAAATTAGTTATG | TTAA | TAAAATTGTTAAAGATGGTGATGAA |
“ | pPV356 | PV2-3 | JX070932 | 75336 | NDb | 3211 | TTCGATATCTCTTGTTACTTTTCAA | TTAA | AAAAAAAAATATTTTTTAAGGCAAA |
PV3 | pPV356 | PV3-1 | JX070933 | 75034 | NDb | 3303 | GTATCCAAAGTGCTTGTTATATTAC | TTAA | AAAGTTAATGGAAGCATAATAAATT |
PV4 | pPV254 | PV4-1 | JX070934 | 75488 | I | 603672 | ATAAGAACACGTCCTAAGTGAAATC | TTAA | TGCTGTTCGAGGATGAAAATTTTCA |
Contig identities, insertion locations within contigs and, where possible, chromosome numbers are derived from the draft S.ratti genome sequence.
Chromosome.
Not determined.