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. 2012 Aug 9;8(8):e1002871. doi: 10.1371/journal.ppat.1002871

Table 2. Genomic integration sites for reporter transgenes and their flanking sequences in three stable transgenic lines of Strongyloides ratti.

Stable line Donor vector Insertion Integration junction accession Contig Chr.a Location 5′ sequence junction Target site 3′ sequence junction
PV2 pPV356 PV2-1 JX070935 74278 I 172601 ACTTTAACGTTGTCATGATTTTTTT TTAA ATTTCATTATACTATTTTAAAAGTA
pPV356 PV2-2 JX070931 74996 I 155901 GGTGGTGGAGATAAAATTAGTTATG TTAA TAAAATTGTTAAAGATGGTGATGAA
pPV356 PV2-3 JX070932 75336 NDb 3211 TTCGATATCTCTTGTTACTTTTCAA TTAA AAAAAAAAATATTTTTTAAGGCAAA
PV3 pPV356 PV3-1 JX070933 75034 NDb 3303 GTATCCAAAGTGCTTGTTATATTAC TTAA AAAGTTAATGGAAGCATAATAAATT
PV4 pPV254 PV4-1 JX070934 75488 I 603672 ATAAGAACACGTCCTAAGTGAAATC TTAA TGCTGTTCGAGGATGAAAATTTTCA

Contig identities, insertion locations within contigs and, where possible, chromosome numbers are derived from the draft S.ratti genome sequence.

a

Chromosome.

b

Not determined.