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. 2012 Aug 9;8(8):e1002878. doi: 10.1371/journal.pgen.1002878

Figure 4. The loss of dMi-2 function promotes chromosome compaction.

Figure 4

Third-instar UAS-dMi-2 Δ932-1158 6-5/His2Av-mRFP1; +/da-GAL4 tubP-Gal80ts larvae were shifted from 18°C to 29°C to activate dMi-2 Δ932-1158 expression. (A and B) Live analyses of salivary gland nuclei of UAS-dMi-2 Δ932-1158 6-5/His2Av-mRFP1; +/da-GAL4 tubP-Gal80ts larvae maintained at 18°C (A; n = 11) or shifted to 29°C for 48 hours (B; n = 14). (C–E) Quantification of the volume (C), His2Av-mRFP1 fluorescence (D), and compaction (E) of salivary gland chromosomes of larvae maintained at 18°C or 29°C for 48 hours after the temperature shift. (F) Scatter plot of the data used to generate C–E. Chromatin compaction vs. DNA content are compared for single nuclei of larvae maintained at 18°C (green triangles) or 29°C (purple circles) for 48 hours. A and B scale bars are 10 µm.