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. Author manuscript; available in PMC: 2012 Aug 9.
Published in final edited form as: Cell Signal. 2010 Mar 7;22(7):1063–1075. doi: 10.1016/j.cellsig.2010.02.009

Figure 5. p38 MAPK activation alters the subcellular localization of MK5-V5 variants.

Figure 5

HEK293 cells were transfected with (A) pIRES-MK5.1-V5-EGFP, (B) pIRES-MK5.2-V5-EGFP, (C) pIRES-MK5.3-V5-EGFP, (D) pIRES-MK5.4-V5-EGFP, (E) pIRES-MK5.5-V5-EGFP, or (F) pIRES-MK5.1-T182A-V5-EGFP. After 24 h of transfection cells were serum starved for 6 h and then treated with or without anisomycin (15 μg/ml, 2 h), SB203580 (20 μM), or LMB (3 ng/ml, 10 min). Where indicated, cells were preincubated with SB203580 for 10 min prior to the addition of anisomycin. Following treatment, cells fixed and MK5 was visualized by staining with an anti-V5 antibody and Alexa 555-coupled secondary (anti-mouse) antibody (upper panels). Nuclei were visualized by TO-PRO 3 iodide (1.5 μM) staining (lower panels). Several fields of cells were examined and representative images are shown.