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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: IUBMB Life. 2012 May 28;64(8):684–689. doi: 10.1002/iub.1041

Figure 1. Fluorescent proteins created by in vitro (conventional) and in vivo (recombineering) site-directed mutagenesis.

Figure 1

Mostaza and Azure FP were created by in vitro site-directed mutagenesis while all others were created by recombineering. (A) Confocal image of Aqua, GFPmut3* and Amarillo E. coli. (B) Excitation and emission spectra of fluorescent protein extracts.