Fig 8.

Expression of all1711 in the wild type, two all1711 mutants, and corresponding complemented all1711 mutant strains. Northern blot analysis was performed with the all1711 probe indicated in Fig. 1A and RNA from cells grown in bubbled BG11C medium containing Nm for FQ163 and FQ296 and Nm plus Em for the complemented mutants (see genotypes in Table 1). The approximate sizes of different all1711 transcripts are indicated. Because the gene is expressed from a set of promoters in the plasmid complementing the mutants that differ from the promoter in the wild-type chromosome, the observed sizes differ from that in Fig. 3. Hybridization with a probe of the Anabaena rnpB gene was used as a loading and transfer control (lower panel).