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. 2012 Sep;194(17):4698–4708. doi: 10.1128/JB.00225-12

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Fig 3 — In vitro autophosphorylation activity of GST-Nla28S-cyt. (A) Time course of the autophosphorylation activity of GST-Nla28S-cyt (GST-Nla28S-cyt∼P) incubated with [γ-³²P]ATP at room temperature. (B) Autophosphorylation activity of GST-Nla28S-cyt incubated with [γ-³²P]ATP for 60 min and treated with 0.1 N HCl or 1 N NaOH for 20 min at room temperature. (C) Autophosphorylation activity of GST-Nla28S-cyt, GST-Nla28S-cyt H242A, and GSTNla28S-cyt D386A incubated with [γ-³²P]ATP for 60 min at room temperature.