Fig 4.

In vitro phosphotransfer assays. (A) GST-Nla28 was incubated with [γ-³²P]ATP or [³²P]acetyl phosphate ([³²P]AcPO₄) for 30 min. (B) Autophosphorylated GST-Nla28S-cyt (GST-Nla28S-cyt∼³²P) was incubated with GST-Nla28 at room temperature. (C) Autophosphorylated GST-Nla28S-cyt was incubated with His-OmpR or in kinase buffer alone at room temperature for 60 min. (D) Autophosphorylated His-EnvZ-cyt was incubated in kinase buffer alone, with GST-Nla28, or with His-OmpR at room temperature for 60 min. (E) Autophosphorylated GST-Nla28S-cyt was incubated in kinase buffer alone, with GST-Nla28, or with GST-Nla28 D53A at room temperature for 60 min.