Strains |
|
|
M. xanthus
|
|
|
DK1622 |
Wild type |
13a |
DK4398 |
asgB |
19a |
AG1400 |
DK1622 Δnla28S
|
This study |
AG1401 |
Δnla28S + nla28S (pZS202) |
This study |
AG1402 |
Δnla28S + nla28S H242A (pZS210) |
This study |
E. coli
|
|
|
TOP10 |
Cloning host |
Invitrogen |
BL21(DE3) |
Expression host |
Lab collection |
NiCO21(DE3) |
Expression host |
New England Biolabs |
Plasmids |
|
|
pCR-Blunt |
Cloning vector, Kanr
|
Invitrogen |
pGEX-4T3 |
GST fusion expression vector, Ampr
|
GE Healthcare |
pET28b |
His tag fusion expression vector, Kanr
|
EMD Biosciences |
pBJ114 |
Plasmid used for gene replacements and deletions, Kanr
galK+
|
47 |
pZS93b |
nla28S deletion cassette in pCR-Blunt |
This study |
pZS93 |
nla28S deletion cassette in pBJ114 |
This study |
pZS108b |
nla28S-cyt in pCR-Blunt |
This study |
pZS109b |
nla28 in pCR-Blunt |
This study |
pZS108 |
nla28S-cyt in pGEX-4T3 |
This study |
pZS109 |
nla28 in pGEX-4T3 |
This study |
pZS134b |
envZ-cyt in pCR-Blunt |
This study |
pZS135b |
ompR in pCR-Blunt |
This study |
pZS134 |
envZ-cyt in pET28b |
This study |
pZS135 |
ompR in pET28b |
This study |
pZS187 |
nla28S-cyt H242A in pGEX-4T3 |
This study |
pZS188 |
nla28S-cyt D386A in pGEX-4T3 |
This study |
pZS191 |
nla28- D53A in pGEX-4T3 |
This study |
pZS202 |
1,000-bp upstream region + nla28S in pCR-Blunt |
This study |
pZS210 |
1,000-bp upstream region + nla28S H242A in pCR-Blunt |
|