Table 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant feature | Source or reference |
|---|---|---|
| Strains | ||
| M. xanthus | ||
| DK1622 | Wild type | 13a |
| DK4398 | asgB | 19a |
| AG1400 | DK1622 Δnla28S | This study |
| AG1401 | Δnla28S + nla28S (pZS202) | This study |
| AG1402 | Δnla28S + nla28S H242A (pZS210) | This study |
| E. coli | ||
| TOP10 | Cloning host | Invitrogen |
| BL21(DE3) | Expression host | Lab collection |
| NiCO21(DE3) | Expression host | New England Biolabs |
| Plasmids | ||
| pCR-Blunt | Cloning vector, Kanr | Invitrogen |
| pGEX-4T3 | GST fusion expression vector, Ampr | GE Healthcare |
| pET28b | His tag fusion expression vector, Kanr | EMD Biosciences |
| pBJ114 | Plasmid used for gene replacements and deletions, Kanr galK+ | 47 |
| pZS93b | nla28S deletion cassette in pCR-Blunt | This study |
| pZS93 | nla28S deletion cassette in pBJ114 | This study |
| pZS108b | nla28S-cyt in pCR-Blunt | This study |
| pZS109b | nla28 in pCR-Blunt | This study |
| pZS108 | nla28S-cyt in pGEX-4T3 | This study |
| pZS109 | nla28 in pGEX-4T3 | This study |
| pZS134b | envZ-cyt in pCR-Blunt | This study |
| pZS135b | ompR in pCR-Blunt | This study |
| pZS134 | envZ-cyt in pET28b | This study |
| pZS135 | ompR in pET28b | This study |
| pZS187 | nla28S-cyt H242A in pGEX-4T3 | This study |
| pZS188 | nla28S-cyt D386A in pGEX-4T3 | This study |
| pZS191 | nla28- D53A in pGEX-4T3 | This study |
| pZS202 | 1,000-bp upstream region + nla28S in pCR-Blunt | This study |
| pZS210 | 1,000-bp upstream region + nla28S H242A in pCR-Blunt |