Skip to main content
. 2012 Sep;194(17):4570–4578. doi: 10.1128/JB.00661-12

Fig 4.

Fig 4

BpaB binds cp32 DNA 5′ of nucP and ssbP. (A) EMSA of BpaB with labeled DNA probes consisting of sequence 5′ of the cp32-1 nucP ORF (probe B-3.1), the cp32-1 erpAB operator, and the chromosomal flaB ORF. Lane 1 contained only DNAs. Lanes 2 to 5 also contained recombinant BpaB321 at concentrations of 0.03, 0.06, 0.09, 0.12, and 0.15 μM. Densitometric analyses indicated that BpaB binds the site 5′ of nucP with an approximately 1.4-fold-greater affinity than the site in the erpAB operator. (B) EMSA of BpaB, labeled nucP 5′ noncoding DNA (probe B-3.1), and unlabeled competitor DNAs. Lane 1 contained probe B-3.1 only, while the other lanes also included 0.1 μM BpaB321. Lanes 3 to 6, 7 to 10, and 11 to 14 additionally contained unlabeled probes at molar concentrations that were 63-, 125-, 250-, and 500-fold greater than that of the labeled probe. (C) EMSA indicating binding of BpaB to DNA 5′ of ssbP. Lane 1 contained only labeled ssbP DNA (probe B-4), while lanes 2 to 7 contained BpaB321 at concentrations of 0.3, 0.6, 0.9, 1.2, 1.4, and 1.5 μM.