Fig 3.

(A) Schematic representation of SltF showing the Sec signal domain, the transglycosylase (SLT) domain, the C terminus domain (CT), and the three C-terminus deletions. (B) Swimming assays on soft agar plates were carried out in 0.25% agar supplemented with 100 μM sodium propionate. The strains analyzed included WS8 (wild-type), SltF1 (ΔsltF), SltF1/sltF, SltF1/sltFΔ4, SltF1/sltFΔ5, and SltF1/sltFΔ6. The various alleles of sltF were cloned in pRK415 (for details, see Materials and Methods). (C) Zimodot analysis of wild-type SltF and the three C-terminal mutant proteins. Lysozyme activity was included as a control. (D) Western blot analysis with anti-SltF gamma globulins (1:2,500 dilution) of the same strains shown in panel B. (E) Western blot analysis of the same strains with anti-flagellin gamma globulin (1:10,000 dilution). SDS–12.5% PAGE was used in all cases.