Table 1.

Cyanuric acid hydrolase/barbiturase family proteins identified in this study and identities to enzymes of known function

Clade and organisma	GI no.	Locus name	% Identity to:
			AtzD	TrzD	Moorella cyanuric acid hydrolase	Barbiturase
1
Clostridium asparagiforme DSM 15981	225388419	CLOSTASPAR_02155	51	57	65	45
Moorella thermoacetica ATCC 39073	83590946	Moth_2120	58	63	100	46
Paenibacillus sp. JDR-2	251798893	Pjdr2_4927	50	55	58	43
Bacillus cellulosilyticus DSM 2522	317127947	Bcell_1233	37	42	41	33
Acidovorax citrulli strain 12227	3659839		58	100	63	45
Enterobacter cloacae strain 99	13022197		58	100	63	45
Aminobacter aminovorans (formerly Comamonas sp. A2)	14029763	Partial sequence	58	100	63	45
Bradyrhizobium japonicum USDA 110	27382392	blr7281	59	55	59	42
Pseudomonas sp. ADP	32455884		100	58	58	43
Pseudomonas nitroreducens	285310188		100	58	58	43
Comamonas sp. A2	254972448		100	58	58	43
Arthrobacter sp. MCMB-436	47059636		100	58	58	43
Arthrobacter AD25	117583156		99.7	58	58	43
Micromonas sp. strain RCC299	255079800	MICPUN_59838	45	41	42	32
Acidithiobacillus ferrooxidans ATCC 53993 and ATCC 23270	198283056 218519570	Lferr_0922 AFE_0779	42	46	46	34
Methylobacterium radiotolerans JCM 2831	170751160	Mrad2831_4776	51	55	51	42
Oceanicola granulosus HTCC2516	89068460	OG2516_12979	44	44	44	37
Agrobacterium vitis S4	222106665	Avi_5653	48	50	56	43
Rhizobium leguminosarum bv. viciae 3841	116254793	pRL100353	48	52	54	43
Rhizobium leguminosarum bv. trifolii WSM1325	241554234	Rleg_6452	48	52	53	44
Methylobacterium sp. 4-46	170741972	M446_3816	46	47	47	37
Bradyrhizobium sp. strain BTAi1	148252873	BBta_1315	47	47	52	38
Bradyrhizobium sp. strain ORS278	146343122	BRADO6319	47	48	54	40
Azorhizobium caulinodans ORS 571	158425516	AZC_3892	50	52	55	41
2
Nocardioides JS614	119718489	Noca_4270	43	44	45	72
Thermobispora bispora DSM 43833	296270184	Tbis_2213	43	49	50	77
Streptosporangium roseum DSM 43021	271967801	Sros_6541	42	46	48	74
Geodermatophilus obscurus DSM 43160	284988804	Gobs_0181	42	46	45	80
Catenulispora acidiphila DSM 44928	256391076	Caci_1879	45	47	47	81
Nakamurella multipartita DSM 44233	258655427	Namu_5328	43	45	46	84
Nocardioides sp. strain JS614	119715741	Noca_1505	43	46	46	86
Intrasporangium calvum DSM 43043	315588294	Intca_1068	43	47	48	80
Janibacter sp. strain HTCC2649	84497968	JNB_17813	42	45	45	85
Rhodococcus erythropolis JCM 3132	46395595		43	45	46	100
Rhodococcus erythropolis SK121	229318388	RHOER0001_3381	43	45	46	99.7
Rhodococcus erythropolis PR4	226309369	RER_58820	43	45	46	99.7
Rhodococcus opacus B4	226362213	ROP_27990	42	46	45	92
Rhodococcus jostii RHA1	111022263	RHA1_ro05296	45	46	45	93
Saccharomonospora viridis DSM 43017	257056105	Svir_20970	44	46	47	85
Azorhizobium caulinodans ORS 571	158424827	AZC_3203	49	48	52	43
Frankia sp. EuI1c	280963003	FraEuI1c_3137	36	36	40	38

^aOrganisms containing homologs are in the same order as in the phylogenetic tree in Fig. 2. Organisms in which proteins were experimentally assayed are highlighted in bold.