Table 2.
Kinetic constants of various cyanuric acid hydrolase/barbiturase family enzymes, using cyanuric acid as a substrate
| Enzyme name and /or organism (reference) | kcat (s−1) | Km (μM) | kcat/Km (s−1 M−1) |
|---|---|---|---|
| TrzD (20) | 250 | 50 | 5 × 106 |
| TrzD (this study) | 14.2 ± 0.4 | 58 ± 7 | (2.5 ± 0.4) × 105 |
| Pseudomonas sp. ADP AtzD (12) | 6.8 ± 0.7 | 57 ± 10 | (1.2 ± 0.3) × 105 |
| Pseudomonas sp. ADP AtzD (this study) | 73 ± 6 | 23 ± 7 | (3.2 ± 1.2) × 106 |
| Moorella thermoacetica ATCC 39073 (23) | 10.6 | 110 | 1.0 × 105 |
| Bradyrhizobium japonicum USDA 110 | 9.3 ± 0.7 | 50 ± 10 | (1.9 ± 0.5) × 105 |
| Rhizobium leguminosarum bv. viciae 3841 | 5 ± 1 | 130 ± 60 | (3.8 ± 2.5) × 104 |
| Methylobacterium sp. 4-46 | 17 ± 2 | 69 ± 16 | (2.5 ± 0.9) × 105 |
| Azorhizobium caulinodans ORS 571 locus AZC_3892 | 50 ± 9 | 370 ± 90 | (1.3 ± 0.6) × 105 |
| Azorhizobium caulinodans ORS 571 locus AZC_3203 | —a | — | — |
a
—, no activity with any substrate tested.