Fig 6.

Cells grown in the presence of sublethal concentrations of daptomycin have a striking change in morphology. (A to F) Images of B. subtilis (PY79) after 2 h of growth in the presence of a sublethal concentration of daptomycin (1 μg/ml). The cell membranes are stained red with FM 4-64. (G to J) Daptomycin (Dap)-BODIPY localizes to membrane patches and to the membrane at the interiors of curved regions at sublethal concentrations. The panels show membrane staining (top), daptomycin-BODIPY labeling (bottom), or both (middle). PY79 was grown in LB with the sublethal dose of 10 μg/ml daptomycin-BODIPY for 2 h at 37°C. All panels are at the same scale. Scale bars, 1 μm.