Fig 5.

Accumulation of choline and GB in a set of Proteobacteria capable of aerobic choline catabolism as measured by ¹³C-NMR when the organisms were grown on choline as a sole carbon source. P. aeruginosa (P.a.) and Burkholderia cepacia (B.c.) were grown at both 30°C and 37°C. P. putida (P.p.), P. fluorescens (P.f.), P. syringae (P.s.), and Sinorhizobium meliloti (S.m.) were grown at 30°C. Panel B is identical to Fig. 1A and is our representative image for this condition in P. aeruginosa. Data are representative of at least three independent experiments. Abbreviations: c, choline; g, glycine betaine; me, [¹³C]methanol. Results in each panel are representative of at least three experiments.