Table 1.
Choline and GB pool quantificationa
| NaCl concn (mM) | Carbon source(s)b | Choline pool (nmol/mg [dry wt]) (±SD) | GB pool (nmol/mg [dry wt]) (±SD) |
|---|---|---|---|
| 50 | Choline | 42.11 (29.42) | 56.23 (16.27) |
| Pyruvate + choline | 23.57 (2.64) | 112.54 (35.03) | |
| Glucose + choline | 13.12 (1.44) | 52.70 (13.34) | |
| Citrate + choline | 16.52 (5.59) | 46.05 (12.41) | |
| 750 | Cholinec | 66.61 (26.42) | 51.26 (15.24) |
| Pyruvate + choline | 36.39 (9.33) | 20.69 (1.34) | |
| Glucose + choline | 14.22 (7.70) | 166.07 (49.14) | |
| Citrate + choline | 81.15 (15.58) | 44.65 (31.14) |
a
Normalized to the methanol standard and quantified as described in Materials and Methods.
b
Carbon sources are for a total of 20 mM. In all cases, [13C]choline was added to 5 mM.
c
Calculations for pool sizes reflect that only 25% of the supplied choline is [13C]choline.