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. Author manuscript; available in PMC: 2013 May 23.
Published in final edited form as: J Am Chem Soc. 2012 Apr 10;134(20):8543–8550. doi: 10.1021/ja3004655

Figure 3.

Figure 3

Pressure sensitivity of fast methyl-bearing side chain motion in human ubiquitin. Representative plots of the pressure dependence of the squared generalized order parameter of the methyl symmetry axis for methyl probes in ubiquitin. Examples include those showing a linear (L8δ1, I61δ1, L67δ1, L71δ1) or statistically significant higher order polynomial dependence (T14γ2, V17γ1) upon applied hydrostatic pressure. Fitted Taylor series parameters (Equation 4) and tests for statistical significance are summarized in Table S4. For those sites that have a linear dynamical response to pressure and are not involved in significant structural change (rmsd < 3 Å) (Table S5), the pressure dependence of the methyl dynamics (dO2axis/dP) and the amplitude of motion (O2axis) at ambient pressure are linearly correlated (Figure 4).