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. Author manuscript; available in PMC: 2013 Jun 6.
Published in final edited form as: J Am Chem Soc. 2012 May 24;134(22):9150–9153. doi: 10.1021/ja3041702

Figure 1.

Figure 1

(A) HK is activated by an extracellular signal, which results in phosphorylation of a conserved histidine by ATP. The phosphoryl group is transferred to the aspartate on a RR. Activated RR then triggers a response, typically acting as a transcription factor. Domains: DHp, dimerization and histidine phosphotransfer; CA, catalytic and ATP-binding. (B) Mechanisms of HK autophosphorylation and phosphotransfer to RR. (C) Structure of B-ATPγS, highlighting the thiophosphate-BODIPY moiety that is transferred during autophosphorylation. (D) Proposed mechanism of B-ATPγS labeling of active HK and cognate RR.