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(A) His-tagged HK853 was conjugated to NiNTA-bearing liposomes and separated into free and bound HK853. (B) 600 ng of HK853 from purified stock solution (S), free HK853 after ultracentrifugation (F), and liposome-bound HK853 (B) was labeled with 2 µM B-ATPγS. Inhibition of fluorescent labeling was achieved by the addition of ATP prior to B-ATPγS. Use of B-ATPγS reveals that HK853 bound to liposomes is more active than that free in solution.
