Fig. 3.

GF mice have a decreased frequency of CD4 T cells and osteoclast (OCL) precursor cells (CD11b⁺/Gr1^–) in bone marrow. Femur bone marrow cells from 7-week-old GF and CONV-R female mice were stained with antibodies recognizing CD8, CD4, CD11b, and Gr1 and analyzed by flow cytometry, gating as shown. (A) Values represent the percentage of CD8⁺ and CD4⁺ cells in the total bone marrow population. Data are representative of two independent experiments. (B) Values represent the percentage of CD11b⁺/Gr1^– cells (OCL precursor cells; see text for details) in the total bone marrow population. Data are representative of two independent experiments.