Table 1.

Effects of pre-treatment (i.c.v.) with ODNs (for Gα_i1, Gα_i2, Gα_i3, Gα_o, Gα_s or SCR) on the diuretic and natriuretic responses to i.c.v. guanabenz in conscious Sprague-Dawley rats

i.c.v. ODN pre-treatment (24 h; 25 µg)	Cumulative urine output (µL per 150 min)	Peak Δ urine output (µL·min−1)	Peak Δ UNaV (µeq·min−1)
Saline vehicle	10 569 ± 619	136 ± 9	6.2 ± 0.8
Scrambled (SCR)	9 998 ± 496	132 ± 11	5.9 ± 0.6
Gαi1	10 246 ± 513	153 ± 16	6.1 ± 0.8
Gαi2	9 933 ± 361	125 ± 11	0.6 ± 0.3**
Gαi3	10 960 ± 691	143 ± 16	6.2 ± 0.9
Gαo	10 171 ± 514	127 ± 10	6.2 ± 0.5
Gαs	10 654 ± 461	150 ± 13	5.8 ± 0.6

The values are means ± SEM and illustrate the diuretic (expressed as cumulative urine output in µL per 150 min and peak change in urine output in µL·min⁻¹) and natriuretic [expressed as peak change in urinary sodium excretion (UNaV) in µeq·min⁻¹] effects of i.c.v. guanabenz (50 µg) in six conscious rats per group that were pre-treated (24 h) with an ODN (25 µg in 5 µL) or isotonic saline vehicle (5 µL).

^**P < 0.01, compared with SCR ODN pre-treatment group value.