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. 2011 Sep 14;31(37):13224–13235. doi: 10.1523/JNEUROSCI.0976-11.2011

Figure 4.

Figure 4.

Bimolecular fluorescent complementation can be used to monitor different populations of channel heteromers within the cell based on protein compartmentalization. A, Representative confocal images of COS-7 cells transfected with Kv1.4-PHN plus Kv1.4-PHC (1, 2), Kv1.1-PHN plus Kv1.1-PHC (3, 4), Kv1.4 T328A-PHN plus Kv1.4 T328A-PHC (5, 6), or Kv1.4 T328A-PHN plus Kv1.1-PHC (7, 8) either in the absence (left column, −PSD95) or presence (right column, +PSD95) of PSD95. Scale bars, 5 μm. B, Bar graph showing quantification of number of channel clusters per cell for conditions shown in frames 1–8 (n = 3–6 for each condition). Conditions were compared using one-way ANOVA followed by Tukey's posttest. *p < 0.05 when compared with the minus PSD-95 control. C, Representative confocal images of COS-7 cells transfected with Kv1.4ΔTDV-PHN plus Kv1.4Δ-PHC (1, 2), Kv1.4-PHN plus Kv1.4ΔTDV-PHC (3, 4), Kv1.1-PHN plus Kv1.4-PHC (5, 6), Kv1.1 ΔTDV-PHN plus Kv1.4-PHC (7, 8), Kv1.1-PHN plus Kv1.4ΔTDV-PHC (9, 10), or Kv1.1ΔTDV-PHN plus Kv1.4ΔTDV-PHC (11, 12) either in the absence (left column, −PSD95) or presence (right column, +PSD95) of PSD95. Scale bars, 5 μm. D, Bar graph showing quantification of number of channel clusters per cell for conditions shown in frames 1–12 (n = 4–10 for each condition). Conditions were compared using one-way ANOVA followed by Tukey's posttest. *p < 0.05 when compared with the −PSD-95 control. Error bars indicate SEM.