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. 2011 Sep 14;31(37):13224–13235. doi: 10.1523/JNEUROSCI.0976-11.2011

Figure 7.

Figure 7.

Kv1.4–1.2 heteromeric channels, but not Kv1.4 homomeric channels, are clustered at presynaptic sites. A, B, Clusters of heteromeric Kv1.4–Kv1.2 channel colocalize with a presynaptic marker. Hippocampal neurons were transfected with Kv1.4-PHC plus Kv1.2-PHN (A) or Kv1.4-PHN plus Kv1.4-PHC (B), and then fixed, permeabilized, and immunostained with antibodies against GFP to mark transfected cells (red, top right) and Bassoon to mark the presynaptic termini (cyan, bottom left). Fluorescence from complemented pHluorin is shown on top left (green). Merged image is shown on bottom right. The arrowheads denote areas of colocalization between heteromeric channel and bassoon (Pearson's coefficient of 0.676044 for Kv1.4–Kv1.2 vs 0.11486 for Kv1.4–Kv1.4). Scale bar, 20 μm. C, D, Line intensity quantification analysis from images in A and B, respectively. The arrowheads in C represent clusters of colocalization seen in A.