Fig. 3.
Western blot analysis of gene expression in RAR-β2–transfected esophageal cancer cells. TE-1V1, TE-8V1, and TE-3V1 are stable vector only–transfected sublines, whereas TE-1S4, TE-1S16, TE-8S20, and TE-8S22 are RAR-β2–transfected sublines and TE-3A3 and TE-3A5 are antisense RAR-β2–transfected sublines of TE-1, TE-3, and TE-8, respectively. The cells were maintained in 200 µg/mL G418, and total cellular protein was extracted and subjected to Western blot analysis of COX-2 expression.