Table 3.

Differentially Expressed Genes in the stp1 Deletion Mutant^a Compared to Its Parent Strain^b

ORF IDc	Gene	Description	Fold Changed
Downregulated genes
Virulence
SACOL1187	PSM β2	Phenol-soluble modulin	1.9
SAS065	hld	δ-hemolysin	1.9
SA0387	set11	Superantigen-like protein	1.55
Unknown function
SAP007		Hypothetical protein	2.1
SAS059		Hypothetical protein	1.64
Upregulated genes
Cell wall metabolism
SA1103	uppS	Undecaprenyl pyrophosphate synthetase	1.77
SA1898	sceD	Transglycosylase	1.86
Other cellular function
SACOL1053	yfbB	Acyl-CoA thioester hydrolase	2.37
SA2186	nasF	Uroporphyrin-III C-methyl transferase	2.16
SA1082	rimM	16S ribosomal RNA processing protein	1.69
SA1184	citB	Aconitate hydratase	1.62
SA2180	nreB	Sensor histidine kinase	1.61
SA0587	mntC	Manganese ABC transporter permease	1.53
SA1421		Putative metal-dependent phosphohydralase	1.52
Unknown function
SACOL1331		Hypothetical protein	2.9
SACOL2365		Hypothetical protein	2.15
SA0287		Hypothetical protein	2.09
SA0289		Hypothetical protein	1.96
SAR0592		Hypothetical protein	1.58
SA0336		Hypothetical protein	1.56

Abbreviations: ABC, adenosine triphosphate binding casette; ID, identification; ORF, open reading frame; PSM, phenol-soluble modulin.

^a The cotranscribed kinase, pknB, showed no significant change in expression between the stp1 deletion mutant and its parent strain (A5937).

^b Differential expression was confirmed using quantitative reverse-transcriptase polymerase chain reaction

normalized to 16S ribosomal RNA expression for hld (downregulated 4.07 [±1.0]-fold), PSM β2 (downregulated 2.31 [±0.7]-fold), and sceD (upregulated 1.51 [±0.2]-fold).

^c ORF IDs derived from N315 (SA/SAP/SAS), COL (SACOL), and methicillin-resistant Staphylococcus aureus MRSA252 (SAR) genome annotations.

^d Genes with fold change of ≥1.5 with adjusted P value of ≤.05 were included.