Table 1.
Effect of temperature and dilution on the change (%change) of various coagulation parameters to rFVIIa
| 0 dilution | 40% LR | 40% Hex | ||||
|---|---|---|---|---|---|---|
|
| ||||||
| 37°C | 34°C | 37°C | 34°C | 37°C | 34°C | |
| Thrombin Generation | ||||||
| Lagtime (minutes) | -1.8±0.1 (-53±10) | -2.4±0.6 (-82±6) | -2.6±0.4 (-65±7) | -1.9±0.4 (-78±7) | -2.5±0.4 (-75±8) | -2.0±0.3 (-84±5) |
| ttPeak (minutes) | -2.9±0.7 (-20±4) | -6.1±1.2 (-42±6) | -3.9±0.7 (-25±4) | -3.8±0.6 (-33±3) | -3.9±0.8 (-28±2) | -4.6±0.5 (-40±3) |
| Peak (RFU/min) | 150±31 (21±5) | 579±74* (85±20)* | 49.5±22.2 (10±4) | 288±66* (26±7) | 105.5±32.7 (15±6) | 360±46* (35±7) |
| ETP | 1297±388 (10±3) | 3777±496* (25±5)* | 181±278 (2±2) | 1196±496 (5±2) | 1315±385 (9±3) | 2207±173 (11±1) |
| TEG | ||||||
| R (minutes) | -3.6±0.9 (-69±11) | -5.3±1.9 (-88±23) | -2.0±0.5 (-45±6) | -3.6±1.0 (-59±9) | -2.9±1.0 (-51±12) | -4.9±1.9 (-70±21) |
| K (minutes) | -1.2±0.1 (-69±5) | -1.1±0.7 (-64±24) | -0.2±0.3 (-15±11) | -0.6±0.3 (-25±11) | -2.2±0.7 (-84±36) | -2.0±1.3 (-55±35) |
| α angle (degrees) | 12.3±1.0 (19±2) | 12.7±3.7 (20±7) | 3.6±2.5 (5±5) | 6.1±0.6 (11±4) | 11.3±3.6 (22±6) | 7.7±3.6 (15±8) |
| MA (mm) | 6.8±1.2 (11±2) | 4.5±2.1 (7±4) | 2.2±1.1 (4±2) | 1.9±0.6 (4±1) | 7.8±2.5 (16±4) | 2.2±1.9 (4±4) |
| PT (seconds) | -0.2±0.3 (-2±3) | -1.4±0.5 (-12±4) | -2.3±1.1# (-15±10) | -3.7±0.3 (-27±1) | -2.5±0.4# (-20±2) | -2.5±0.3 (-20±2) |
| aPTT (seconds) | -1.7±0.8 (-6±3) | -4.1±1.3 (-12±4) | -2.9±1.5 (-9±5) | -4.8±0.5 (-14±1) | -4.1±0.4 (-13±1) | -3.8±0.3 (-12±1) |
Values represent Mean±SEM of the change to the addition of rFVIIa;
*
=P<0.05 as compared to 37°C at that dilution;
#
=P<0.05 as compared to 37°C, 0 dilution.