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. Author manuscript; available in PMC: 2013 Jun 8.
Published in final edited form as: J Mol Biol. 2012 Mar 21;419(3-4):223–233. doi: 10.1016/j.jmb.2012.03.010

Fig. 4.

Fig. 4

Homodimerization energetics and kinetic parameters for biotin transfer to BCCP. A. Gibbs free energies of homodimerization. B. The kcat/KM values for biotin transfer to BCCP87 obtained from steady-state measurements. *Not Determined. C. The bimolecular rates of association, k1, of variants with BCCP87 obtained from single turnover kinetic measurements. The kinetic parameters obtained for B and C are shown on a natural logarithmic scale to place them on an energy scale. Error bars for each graph represent the 68% confidence intervals. The bar color coding in each panel is wt BirA (white) and variable loop 140–146 (black), constant loop 170–176 (medium-gray), variable loop 193–199 (dark gray) and variable loop 280–283 (light gray).