Table 1.
Quantitative real-time RT-PCR analyses of gene expression by PCTs and APCTs in relation to expression in normal B-cell subsets*
| Gene | Expression in normal B-cell subsets†
|
Fold change‡ (APCT/PCT) | |||
|---|---|---|---|---|---|
| Naïve | Plasma cell | GC cell | Memory cell | ||
| Spib | X | X | X | 20.06 | |
| Pax5 | X | X | X | 11.91 | |
| Sell | X | 5.93 | |||
| Irf8 | X | X | X | 4.72 | |
| Aicda | X | 4.29 | |||
| Klf2 | X | X | 2.85 | ||
| Ccnd3 | X | 2.75 | |||
| Casp3 | X | X | 2.42 | ||
| Relb | X | X | 1.95 | ||
| Mta3 | X | X | X | 1.92 | |
| Cd80 | X | 1.75 | |||
| Nfkb2 | X | X | 1.68 | ||
| Ets1 | X | X | 1.61 | ||
| Ung | X | X | 1.31 | ||
| Nfkb1 | X | X | 1.17 | ||
| Bcl2 | X | 1.16 | |||
| Runx3 | X | −1.27 | |||
| Rb1 | X | −1.36 | |||
| Ssbp3 | X | −1.85 | |||
| Foxp1 | X | X | −2.03 | ||
| Pml | X | −2.20 | |||
| Bcl6 | X | −2.37 | |||
| Pscdbp | X | X | X | −2.37 | |
| Il6ra | X | X | −4.44 | ||
| Xrcc3 | X | −8.65 | |||
| Atf6 | X | −8.88 | |||
| Bmi1 | X | −9.25 | |||
| Sdc1 | X | −23.07 | |||
| Irf4 | X | −26.13 | |||
| Xbp1 | X | −45.63 | |||
*
Gene transcript levels were determined by qPCR for the 96 genes listed in the Supporting information, Supplementary Table 2 in studies of ten APCTs and 10 PCTs. Data are for genes that differed significantly in expression (p < 0.05) between the two PCN subsets.
†
Expression patterns from ref 24.
‡
Fold change.