Table 2.

Immunoglobulin gene variable region sequences of primary APCTs and cell lines derived from primary APCTs^*

Primary APCT pathology number	Immunoglobulin heavy chain					Immunoglobulin light chain
	V	D	J	C	% of GL	V	J	C	% of GL
36975	J606.4.82	DSP2.7	4	ND	92.7	kh4	2	Kappa	95.4 and 95.8–both stop
	7183.7b	DSP2.9	4	ND	92.4–stop	ba9	2	Kappa	95.6
						VL1	1	Lambda	98.3
37891	36–60.a1.85	DQ52	3	IgM	97.8	kb4	4	Kappa	98.8
						kb4	4	Kappa	98.1–different
						VL2	2	Lambda	99.6
38006	DQ52.8.22	DFL16.1j	4	IgM	100	4.57	5	Kappa	94.4
						VL2	2	Lambda	99.3
38363	J588.20	DSP2.24	4	IgM	99.6	bb1	5	Kappa	100–stop
	J558.20	DSP2.24	4	IgM	100	4–50	5	Kappa	96.9–stop
						VL2	2	Lambda	99.7
38397	DQ52.8.22	DST4.3	3	IgM	99.6	bv9	5	Kappa	99.6
						VL2	2	Lambda	99.3
38400	SM.a2psi.88	DSP2.9	3	IgM	97.7	br9	1	Kappa	100–stop
	DQ52.8.22	DFL16.1j	4	IgM	100	kh4	5	Kappa	98.9
	J558.83.189	DSP2.6	2	IgG	95.9	VL2	2	Lambda	99.7
	Q52.a12.33	DSP2.12	4	IgG	97.8	VL2	2	Lambda	99.7
38696	DQ52.8.22	DFL16.3	3	IgM	99.6	bb1	2	Kappa	100
						kh4	5	Kappa	98.9
						VL2	2	Lambda	99.7
APCT cell line
B6-207	J558.16.106	DFL16.1j	4	IgG	85	ba9	2	Kappa	87.3
B6-1710	J558.12.102	DFL16.1	4	IgG	97.4	23–39	5	Kappa	96.5–stop

^*V(D)J and C region sequences for IgH and IgL. Pathology numbers for primary cases of APCT and cell line designations for cultured lines derived from primary APCTs are listed. Specific V_H, D_H, V_K, and V_L family members identified by sequencing are indicated by name together with J utilization and C region. For individual primary APCTs, cloned sequences that were not repeats of the top sequence listed are also given.

% of GL: per cent identity with germline sequences. Sequences differing by more than 2% from GL are considered to be significantly mutated. Stop: identification of a stop codon blocking expression of the heavy or light chain. ND: not determined as sequencing was done from genomic DNA.