Table 1.

The erCY-RL7 sensor expressed in CHO cells responds to NAC treatment by increase in reduced form

Treatment	Exp. 1	Exp. 2	Exp. 3	Exp. 4	Average	SD
Basal	12.5	13.4	10.9	11.4	12.1	1.1
NAC	31.6	31.3	20.9	28.9	28.2	5.0
Δ	19.1	17.9	10	17.5	16.1	4.1

CHO, Chinese hamster ovary; NAC, N-acetylcysteine

Fractions of erCY-RL7 in reduced form (%) in CHO cells before (basal) and after treatment with 5 mmol/L NAC for 24 hr were determined as described in Materials and methods. Δ Denotes increase in the probe’s reduced form after NAC treatment. Data for each experiment were collected by flow cytometry and represent an average of three replicates collected on 10,000 cells±SD