Table 2.

Comparison of the redox states of ERroGFP1 expressed in CHO cells cultured with and without alteration of intracellular GSH content

Treatment	Exp. 1	Exp. 2	Exp. 3	Exp.4	Exp. 5	Exp. 6	Average	SD
Basal	47.6	54.4	47.5	50.4	38.6	46	47.4	5.2
NAC	64.6	N/A	38.4	51.5	38	39.5	46.4	10.4
BSO	N/A	47	41.4	43.9	39.9	36.5	41.7	3.6

CHO, Chinese hamster ovary; GSH, glutathione; NAC, N-acetylcysteine; BSO, l-buthionine-sulfoximine

Fractions of ERroGFP1-iL in reduced form (%) in cells before (basal) and after treatment with either 5 mmol/L NAC or 0.1 mmol/L BSO (BSO) were determined as described in Materials and methods. Data for each experiment were collected by flow cytometry and represent an average of three replicates collected on 10,000 cells±SD